Development of In vitro Mass Propagation Protocol for Gerbera (Gerbera jamesonii Bolus) var. Orange

نویسندگان

چکیده

An in vitro mass propagation protocol was developed for Gerbera (Gerbera jamesonii Bolus) var. orange using flower bud explants. The young (7 days) explants were found to be suitable culture and highest (100%) callus induction obtained MS medium supplemented with 5.0 mg/l BAP 1.0 NAA. maximum shoot regeneration (95%) the number of shoots (10) observed 6.0 0.3 multiplication frequency (60) only 2.0 BAP. well-developed roots (5.0) per plant average root length (5.0 cm) 100% efficiency without callusing recorded half strength devoid any hormonal supplement. Acclimatized grown plantlets successfully transferred into field condition. Plant Tissue Cult. & Biotech. 32(2): 217-226, 2022 (December)

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Rapid plant regeneration from Gerbera jamesonii Bolus callus cultures

A high frequency shoot organogenesis and plant establishment protocol has been developed for Gerbera jamesonii from ex vitro leaf derived callus. The optimal callus was developed on Murashige and Skoog (MS) basal medium supplemented with 0.4 mg L 6-benzylaminopurine (BAP), 4.0 mg L -naphthalene acetic acid (NAA) and 3% (w/v) sucrose. Two callus types differing in their structures and growth rat...

متن کامل

Rapid in vitro regeneration of Gerbera jamesonii (H. Bolus ex Hook. f.) from different explants

Rapid in vitro multiplication of shoots has been described using capitular sections and leaf explants in Gerbera jamesonii. Three types of basal media regimes were used including MS and two of its modified forms⎯MSI and MSA. Shoot induction was achieved on all three regimes but MSI medium supplemented with Kn (4 mg/l) and IAA (0.5 mg/l) was ideal for shoot bud initiation (8-11) from capitular s...

متن کامل

Tetranychus urticae (Acari: Tetranychidae) on Gerbera jamesonii Bolus and Hook (Asteraceae).

Gerbera (Gerbera jamesonii Bolus and Hook,) is an ornamental Asteraceae of great commercial value, and pests can affect adversely its cultivation. More than 20 species of arthropods cause economic damage on gerbera, among them the two spotted mite, Tetranychus urticae Koch, 1836 (Acari: Tetranychidae), considered a key pest for this and other ornamental plants. In this work, some life-cycle asp...

متن کامل

Flower Development in Gerbera Hybrida, Asteraceae

As a genetically determined structure, flower is an attractive object for developmental studies in plants. Flower development provides a good system for understanding cell differentiation and genetic mechanisms needed for organogenesis. The current molecular view on flower development has been based on studies on relatively few model species, like Arabidopsis and Antirrhinum. Research in model ...

متن کامل

اثر ترکیبات هورمونی مختلف بر ریزازدیادی پانزده رقم ژربرا (Gerbera jamesonii Bolus ex Hooker f.)

ژربرا یکی از مهم‌ترین گیاهان زینتی می‌باشد که به‌صورت گل بریده و گیاه گلدانی مورد استفاده قرار می‌گیرد. روش‌های معمول تکثیر کارایی تامین تقاضای جهانی برای این گیاه زینتی را ندارد. لذا عمومی‌ترین سیستم برای ازدیاد تجاری ژربرا، ریزافزایی می‌باشد. در این پژوهش، اثر تیمارهای هورمونی مختلف و رقم بر تکثیر و ریشه‌زایی ریزنمونه نوک شاخساره پانزده رقم ژربرا مورد بررسی قرار گرفت. در مرحله تکثیر از محیط ک...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Plant Tissue Culture and Biotechnology

سال: 2022

ISSN: ['1818-8745', '1817-3721']

DOI: https://doi.org/10.3329/ptcb.v32i2.63555